Retron Library Recombineering’s RLR technology has some advantages over the popular CRISPR in gene editing, and may soon be used to generate new discoveries
A team from the Wise Institute of Bio-Inspired Engineering at Harvard University announced that it has created a tool that allows scientists to conduct millions of genetic experiments at the same time. The use of RLR (Retron Library Reorganization) technology is called Backtracking It can generate DNA fragments and can overcome some of the limitations found in the most popular tool, CRISPR-Cas9.
The study’s co-author Max Schubert explained: “RLR allows us to do the impossible with CRISPR: we randomly cut the bacterial genome, convert these gene fragments into unique DNA in situ, and then use It is used to analyze millions of simultaneous sequencing. RLR is the simplest and most flexible gene editing tool we can use in complex experiments. It eliminates the common toxicity in CRISPR and improves the ability of researchers to explore genome mutations .” Engadget.
In today’s most commonly used technology CRISPR-Cas9, there are limitations in the transfer of a large amount of material, and the Cas9 enzyme (“molecular scissors”) can also cut the possibility of DNA that should not be cut. On the other hand, this method incorporates the mutant sequence into the genome during the repair process. In RLR, mutated DNA can be introduced into the replicating cell, thereby integrating the sequence into the DNA of the “daughter” cell. Finally, this new method allows you to introduce barcodes or name tags, allowing scientists to monitor individual elements in many bacteria.
The team was able to successfully test the bacteria Escherichia coli And discover antibiotic resistance mutations, this process is much faster than using other tools. RLR has not been fine-tuned, improved, and standardized before it can be used by the entire community, but the creators are confident that it will soon make new and exciting discoveries possible.